IGH
Alternative name:
Immunoglobulin heavy chain
Methodology
FISH
Test Description and clinical significance
Probes
14q32.33
Disease:
Plasma Cell Leukemia; B-cell Acute Lymphocytic Leukemia; Chronic Lymphocytic Leukemia (CLL); Multiple Myeloma; Non-Hodgkin Lymphoma; Mantle Cell Lymphoma; Follicular Lymphoma; Diffuse Large Cell Lymphoma; B-cell Lymphoproliferative Disease
IGH (immunoglobulin heavy chain) is the large polypeptide subunit of an immunoglobulin and located on chromosome 14. FISH can detect the rearrangements of the IGH gene which is associated in many hematologic cancers.
BCR/ABL p190 Minor by PCR is recommended
Specimen Requirements
Collection:
The ordering pathologist will decide whether to order a conventional FISH preparation or a Plasma-cell enrichment (PCE) FISH preparation or a B-cell enrichment (BCE) preparation.
Bone Marrow Aspirate: 1~2mL in Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. Plasma cell enrichment eligible. and B-cell enrichment eligible.
Peripheral Blood: 2~5mL Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. B-cell enrichment eligible. Plasma cell enrichment eligible ONLY IF circulating plasma cells are noted in PB.
Fresh, Unfixed Tissue: Tissue in RPMI. A conventional FISH preparation will be performed as B-cell enrichment and plasma cell enrichment can not be performed on fresh tissues.
Fluids: Equal parts RPMI to specimen volume. A conventional FISH preparation will be performed as B-cell enrichment and plasma cell enrichment can not be performed on fresh fluid tissues.
Paraffin Block: H&E slide and paraffin block. B-cell enrichment can not be performed on paraffin embedded tissues. Plasma cell enrichment can not be performed on paraffin embedded tissues. Enumeration probe types (1p36/1q25, 13q14.2/13q34, CEN9, and TP53/CEN17) are not compatible with paraffin embedded tissues.
Cut Slides: H&E slide and 1 unstained slide (cut at 4 microns) per probe. B-cell enrichment can not be performed on paraffin embedded tissues. Plasma cell enrichment can not be performed on paraffin embedded tissues. Enumeration probe types (1p36/1q25, 13q14.2/13q34, CEN9, and TP53/CEN17) are not compatible with paraffin embedded tissues.
Stability:
120 Hours/48 hours
Unacceptable Conditions:
DO NOT FREEZE
Storage & Transport
Room Temperature/Refrigerated (2–8°C )
CPT(s)
88377
New York Approved
YES
TAT
2 Days
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
