Cytogenetics / FISH

Karyotype (Cytogenetics)

Karyotyping, or chromosomal analysis, can provide insights into diagnosis, prognosis, and prediction of disease in many acute and chronic leukemias and lymphomas. Traditional G-banding for metaphase analysis with specific cell culturing and read out by two independent cytotechnologists ensures optimal results.

  • Best-in-industry turnaround time (3 days)
  • Cell-specific culture (myeloid, B-cell, T-cell)
  • <1% culture failure*

FISH

With its ability to bind to only specific parts of a chromosome and spotlight abnormalities through their tagged fluorescence on cells when viewed under a fluorescence microscope, fluorescence in situ hybridization (FISH, also known as molecular cytogenetics), can identify abnormalities that may be missed at a macro level in karyotype (Cytogenetics), but can captured on a micro level in FISH.

  • Best-in-industry turnaround time (2 days)
  • Disease-focused panels for myeloid, lymphoid, and plasmacytic disorders including cell enrichment
  • Complete suite of hematopoietic FISH probes for custom panels and follow up
  • HER2, ALK, ROS1, MET, RET, 1p/19q and PTEN for solid tumor analysis

Karyotyping for myeloid / lymphoid disorders

Notes
Chromosome Analysis, Bone Marrow and Peripheral Blood, UNSTIMULATED Myeloid 24 and 48 hour cultures

Chromosome Analysis, Bone Marrow and Peripheral Blood, STIMULATED

(T- and B-Cell, Lymphoproliferative Disorders, Plasma Cell)
  • T-Cell 72 hour culture, if necessary
  • B-Cell 120 hour culture, if necessary

*With appropriate cell-specific culture, karyotype (Cytogenetics), and FISH triage.

Hematologic FISH Panels and Probes

ALL FISH Panel
  • BCR/ABL1 t(9;22)
  • KMT2A (11q23)
  • ETV6/RUNX1 t(12;21)
  • IGH-BA (14q32.33)
 AML FISH Panel
  • RUNX1T1/RUNX1 t(8;21)
  • NUP98-BA (11p15.4)
  • KMT2A (11q23)
  • PML/RARA t(15;17)
  • CBFB (16q22)
APL FISH Panel
  • PML/RARA t(15;17)
 Burkitt’s Lymphoma FISH Panel
  • IGH/MYC t(8;14)
  • MYC-BA at 8q24.21
CLL FISH Panel
  • ATM/TP53 (11q22.3/17p13.1)
  • CEN12/D13S319/13q34 (CEN12/13q14.2/13q34)
 CML FISH
  • BCR/ABL1 t(9;22)
Eosinophilia FISH Panel
  • CHIC2 (4q12)
  • PDGFRB (5q32-q33)
  • FGFR1 (8p12)
  • PCM1/JAK2 t(8;9)
 Follicular Lymphoma FISH Panel
  • BCL6 (3q27)
  • MYC-BA (8q24.21)
  • IGH/BCL2 t(14;18)
Mantle Cell Lymphoma FISH
  • CCND1/IGH t(11;14)
 Marginal Zone / MALT Lymphoma FISH Panel
  • CEN3
  • CEN7/MET (7p11.1-q11.1.1/7q31.2)
  • CCND1/IGH t(11;14)
  • BIRC3/MALT1 (11q22.1/18q21.31)
MDS FISH Panel
  • D5S23:D5S721/CSF1R (5p15.2-p15.31/5q32-q33.1)
  • CEN7/MET (7p11.1-q11.1.1/7q31.2)
  • CEN8
  • TP53/CEN17 (17p13.1/17p11.1-q11.1)
  • PTPRT/20q11 (20q12/20q11.21)
 MPN FISH Panel
  • CEN8
  • BCR/ABL1 t(9;22)
  • ETV6(12p13.2)
  • D13S319/13q34 (13q14.2/13q34)
  • PTPRT/20q11 (20q12/20q11.21)
Myeloma FISH Panel
  • 1p36/1q25 (1p36.31/1q25.3)
  • CEN9
  • CCND1/IGH(11q13.3/14q32.33)
  • D13S319/13q34 (13q14.2/13q34)
  • TP53/CEN17 (17p13.1/17p11.1-q11.1)
 Myeloma FISH Reflex panel
  • IGH-BA (14q32.33)
  • FGFR3/IGH (4p16.3/14q32.33)
  • IGH/MAF (14q32.33/16q32.1-q23.2)
  • IGH/MAFB (14q32.33/20q12)

All FISH probes are available individually or in custom panels.

Solid Tumor FISH Panels and Probes

NSCLC FISH Panel
  • ALK (2p23)
  • ROS1 (6q22.1)
  • MET (7q31.2)
  • RET (10q11)
 Glioma Brain Cancer FISH Panel
  • 1p36/1q25 (1p36.31/1q25.3)
  • 19q13/19p13 (19q13.32-q13.33/19p13.3)
  • PTEN/CEN10 (10q23.2-q23.31/10p11.1-q11.1)
HER2 FISH
  • HER-2/neu/CEP17 (17q11.2-12/17p11.1-q11.1)

All FISH probes are available individually or in custom panels.

"While we have many laboratories to choose from, we consider siParadigm to be the most reliable. Contact with a hematopatholgist is rapid and informative."

Barry Kaplan, MD, PhD
Oncologist/Hematologist, Queens Medical Assoc.

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