ATM/TP53
Methodology
FISH
Test Description and clinical significance
Probes
11q22.3/17p13.1
Disease:
CLL, CD5+ B-Cell Lymphoma
ATM: The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates,Tumor Protein P53 This gene encodes a tumor suppressor protein containing transcriptional activation. panel is used to detect chromosome abnormalities (12p11.1-q11/ 13q14.2/13q34) in CLL to guide for prognosis and treatment.
NGS CLL panel and IGHV are recommended
Specimen Requirements
Collection:
The ordering pathologist will decide whether to order a conventional FISH preparation or a B-cell enrichment (BCE) FISH preparation.
Bone Marrow Aspirate: 1~2mL in Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. B-cell enrichment eligible.
Peripheral Blood: 2~5mL Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. B-cell enrichment eligible.
Fresh, Unfixed Tissue: Tissue in RPMI. A conventional FISH preparation will be performed as B-cell enrichment can not be performed on fresh tissues.
Fluids: Equal parts RPMI to specimen volume. A conventional FISH preparation will be performed as B-cell enrichment can not be performed on fresh fluid tissues.
Paraffin Block: H&E slide and paraffin block. B-cell enrichment can not be performed on paraffin embedded tissues.
Cut Slides: H&E slide and 1 unstained slide (cut at 4 microns) per probe. B-cell enrichment can not be performed on paraffin embedded tissues.
Stability:
120 Hours
Unacceptable Conditions:
DO NOT FREEZE
Storage & Transport
Room Temperature
CPT(s)
88377
New York Approved
YES
TAT
2 Days
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
