CD5+ B-Cell Lymphoma FISH Panel
Methodology
FISH
Test Description and clinical significance
Probes
(11q13.3/14q32.33 & 11q22.3/17p13.1 & 12p11.1-q11 & 13q14.2/13q34)
Disease:
B-Cell Lymphoma
Detection of CCND1/IGH translocation. The t(11;14)(q13;q32) translocation results in the CCND1 gene being inserted into the heavy chain (IGH) locus resulting in over production cyclin D1 protein4. IGH-CCND1 Fusion, t(11;14) by FISH is described in many hematologic malignancies. ATM: The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates. Tumor Protein P53:This gene encodes a tumor suppressor protein containing transcriptional activation. TP53 and ATM aberrations both lead to p53 dysfunction. Detection of deletions via fluorescent in situ hybridization (FISH) of TP53 and ATM is part of standardized clinical work-up in CLL. TP53-defective CLL is more aggressive, whereas ATM-defective CLL has a more prolonged clinical course. Used to detect chromosome abnormalities (12p11.1-q11/13q14.2/13q34) in CLL to guide for prognosis and treatment .
Specimen Requirements
Collection:
'1~2ml in Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube/ 10 mL of fluid in RPMI medium, or
1 cm3 marble-sized piece of fresh tumor immersed completely with RPMI medium or sterile saline'
Stability:
120 Hours/48 hours
Unacceptable Conditions:
DO NOT FREEZE
Storage & Transport
Room Temperature/Refrigerated (2–8°C )
CPT(s)
88377x3
New York Approved
YES
TAT
2 Days
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
