Hairy Cell Leukemia (HCL) Flow Panel
Methodology
Flow cytometry
Test Description and clinical significance
Probes/Genes
CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD11c, CD16, CD19, CD20, CD23, CD25, CD38, CD45, CD56, CD57, CD103, CD123, Kappa, Lambda
Disease:
Anemia, leukopenia, leukocytosis, pancytopenia, lymphoma, non-Hodgkin lymphoma, multiple myeloma, plasma cell neoplasm, leukemia, acute leukemia, chronic leukemia
Multiparameter flow cytometry plays an important role in the diagnosis, staging, and monitoring of patients with a suspected hematological malignancy.
Markers run evaluate lymphocytes to identify presence/absence of malignant cells.
Specimen Requirements
Collection:
Peripheral blood: 2-5 mL in Green top (sodium heparin) tube preferred, OR purple top (EDTA) tube
Bone marrow aspirate: 1-2mL in Green top (sodium heparin) tube preferred, OR purple top (EDTA) tube
Fresh tissue: 1 cm3 marble-sized piece of tissue in RPMI or sterile saline
Fluid: 10mL fluid in RPMI
Stability:
Peripheral blood: 48 hours
Bone marrow aspirate: 48 hours
Fresh tissue: 24 hours
Fluid: 24 hours
Unacceptable Conditions:
Specimen submitted is in the incorrect anticoagulant. Specimen received after the stability date. Gross hemolysis. Frozen specimen. Insufficient specimen. Unlabeled. Left un-refrigerated for an extended period.
Storage & Transport
If testing is expected to occur in less than eight hours, specimens must be transported between 18°C and 25°C
If testing is expected to occur in greater than eight hours, specimens must be transported with cold packs to maintain specimens between 2-8°C prior to testing
CPT(s)
88184x1, 88185x20, 88189x1
New York Approved
Yes
TAT
24 hours from specimen receipt
Levels of Service
Global, technical only, professional only
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
