Paroxysmal nocturnal hemoglobinuria (PNH) - bone marrow aspirate panel
Methodology
Flow cytometry
Test Description and clinical significance
Probes/Genes
CD59, CD235a
Disease:
Paroxysmal nocturnal hemoglobinuria (PNH)
Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired somatic mutation in the X linked phosphatidylinositol glycan class A (PIGA) gene, which leaves hematopoietic cells unable to produce the glycosylphosphatidylinositol (GPI) anchor that links cell surface proteins to the plasma membrane.PNH leads to episodic hemolytic anemia, recurrent thrombosis and bone marrow failure and cytopenias /PNH clones frequently appear in patients with aplastic anemia and myelodysplastic neoplasms
Specimen Requirements
Collection:
Bone marrow aspirate: 1-2mL in Green top (sodium heparin) tube preferred, OR purple top (EDTA) tube
Stability:
48 hours
Unacceptable Conditions:
Specimen submitted is in the incorrect anticoagulant. Specimen received after the stability date. Gross hemolysis. Frozen specimen. Insufficient specimen. Unlabeled.
Storage & Transport
Room Temperature
CPT(s)
88184x1, 88185x1, 88187x1
New York Approved
Yes
TAT
24 hours from specimen receipt
Levels of Service
Global
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
