Disease:
Types of HPV can cause cancer means they are High Risk. high-risk type of HPV infects cells, it changes how they communicate with one another. It also causes the cells to multiply. Usually, the immune system becomes aware of these cells and regulates them.

This test provides detection of human papilloma virus E6/E7 mRNA, histological localization of HPV within the tissue, and differentiation of low-risk vs. high-risk subtypes in formalin-fixed paraffin-embedded tissues. Positive results in this assay provide evidence of transcriptional activation of viral E6/E7 oncogenes and support the diagnosis of active infection. Studies have shown RNA ISH to have greater sensitivity and specificity than DNA ISH for HPV detection. RNA ISH may be useful in resolving cases with p16 overexpression that tested negative for HPV DNA by other methods. Testing is commonly performed on tissues of the uterine cervix, anus, and head and neck, particularly the oropharynx. HPV ISH may help resolve cervical cases with morphology discrepant from HPV status as determined from cytology specimens. Positive HPV status is associated with improved overall survival in oropharyngeal squamous cell carcinoma.

Collection:
FFPE/un-stained slides/Fresh tissue in formalin

Stability:
Room temperature

Unacceptable Conditions:
Unlabeled specimen/specimen left unfixed
for extended periods