JAK2 V617F Mutation Analysis reflex to CALR and MPL mutation analysis
Unique Identifier:
ML-H003
Name on Requisition:
JAK2 V617F reflex to CALR*/MPL*
Product Line:
HEME
Panel Group:
PCR
Panel Name:
PCR - MPN- Reflexes
Methodology
Molecular
PCR
Test Description and clinical significance
Genes
JAK2 V617F & CALR & MPL
Disease:
myeloproliferative neoplasm, polycythemia vera, acute myeloid leukemia, overt primary myelofibrosis, and essential thrombocythemia
A polymerase chain reaction assay that detects V617F mutation . JAK2 is a kinase that is misregulated or mutated in a number of myeloproliferative diseases and cancers. The mutation V617F is the most clinically relevant variant, and is seen in around half of myeloproliferative disorders.
Specimen Requirements
Preparation:
PB, BM
Collection:
At least 0.5 ml EDTA, citrate (ACD) or Heparinized whole blood or bone marrow aspirates for DNA mutations
Stability:
96hours/4 to 7 days refrigerated
Unacceptable Conditions:
Specimen submitted is in the incorrect fixative or anticoagulant. Specimen received after the stability date. Gross hemolysis. Frozen specimen. Insufficient specimen. Unlabeled. Left un-refrigerated for an extended period.
Storage & Transport
Blood, bone marrow specimens should be kept at room temperature and preferably received within 96 hrs of drawing. Samples that are 4 to 7 days old should be refrigerated.
CPT(s)
81270, 81219, 81338
New York Approved
Yes
TAT
3
Requisition Type
Heme
Levels of Service
Full
DexS Code
NONE
Prefix
MI
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
