CEN3
Methodology
FISH
Test Description and clinical significance
Probes
3p11.1-q11.1
Disease:
B-cell non Hodgkin lymphoma (NHL), marginal zone B-cell lymphoma
Centromeric probe for chromosome 3. Used to detect chromosome abnormalities (3p11.1-q11.1) in Non hodgkin lymphoma.lymphoma
Specimen Requirements
Collection:
The ordering pathologist will decide whether to order a conventional FISH preparation or a B-cell enrichment (BCE) FISH preparation.
Bone Marrow Aspirate: 1~2mL in Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. B-cell enrichment eligible.
Peripheral Blood: 2~5mL Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. B-cell enrichment eligible.
Fresh, Unfixed Tissue: Tissue in RPMI. A conventional FISH preparation will be performed as B-cell enrichment can not be performed on fresh tissues.
Fluids: Equal parts RPMI to specimen volume. A conventional FISH preparation will be performed as B-cell enrichment can not be performed on fresh fluid tissues.
Paraffin Block: H&E slide and paraffin block. B-cell enrichment can not be performed on paraffin embedded tissues.
Cut Slides: H&E slide and 1 unstained slide (cut at 4 microns) per probe. B-cell enrichment can not be performed on paraffin embedded tissues.
Stability:
120 Hours
Unacceptable Conditions:
DO NOT FREEZE
Storage & Transport
Room Temperature
CPT(s)
88368 for intial single probe
88369 for each additional single probe
New York Approved
YES
TAT
2 Days
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
