JAK2 V617F Mutation analysis reflex to CALR, MPL & all MPN genes
Unique Identifier:
ML-H004
Name on Requisition:
JAK2 V617F reflex to CALR*/MPL* with the entire myeloid panel as clinically indicated
Product Line:
HEME
Panel Group:
PCR/NGS
Panel Name:
PCR - MPN- Reflexes
Methodology
Molecular
PCR, NGS
Test Description and clinical significance
Genes
ABL1, ANKRD26, ASXL1, BCOR, BCORL1, BRAF, CBL, CBLB, CBLC, CDKN2A, CSF3R, CUX1, CXCR4, DDX41, DNMT3A, ETNK1, ETV6, EZH2, FBXW7, FLT3, GATA1, GATA2, GNAS, GNB1, HRAS, IDH1, IDH2, IL7R, JAK1, JAK2, JAK3, KIT, KRAS, MPL, MYD88, NF1, NOTCH1, NPM1, NRAS, PAX5, PHF6, PIGA, PPM1D, PRPF8, PTEN, PTPN11, RB1, RUNX1, SETBP1, SF3A1, SF3B1, SH2B3, SMARCB1, SMC1A, SMC3, SRSF2, STAG2, STAT3, STAT5B, TET2, TP53, U2AF1, U2AF2, UBA1, WT1, XPO1, ZRSR2
Disease:
myeloproliferative neoplasm, polycythemia vera, acute myeloid leukemia, overt primary myelofibrosis, and essential thrombocythemia
A polymerase chain reaction assay that detects V617F mutation . JAK2 is a kinase that is misregulated or mutated in a number of myeloproliferative diseases and cancers. The mutation V617F is the most clinically relevant variant, and is seen in around half of myeloproliferative disorders.
Specimen Requirements
Preparation:
PB, BM
Collection:
At least 0.5 ml EDTA, citrate (ACD) or Heparinized whole blood or bone marrow aspirates for DNA mutations and 3 mL for RNA gene fusions detection
Stability:
96hours/4 to 7 days refrigerated
Unacceptable Conditions:
Specimen submitted is in the incorrect fixative or anticoagulant. Specimen received after the stability date. Gross hemolysis. Frozen specimen. Insufficient specimen. Unlabeled. Left un-refrigerated for an extended period.
Storage & Transport
Blood, bone marrow specimens should be kept at room temperature and preferably received within 96 hrs of drawing. Samples that are 4 to 7 days old should be refrigerated.
CPT(s)
81270, 81338, 81219, IF REFLEX TO FULL PANEL THEN 81455
New York Approved
Yes
TAT
3 to 5
Requisition Type
Heme
Levels of Service
Full
DexS Code
NONE
Prefix
MI
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
