BCR/ABL p210 (major) reflex to BCR/ABL p190 (minor)
Unique Identifier:
ML-H010
Name on Requisition:
BCR/ABL Major Quantitative reflex to Minor
Product Line:
HEME
Panel Group:
PCR
Panel Name:
PCR–CML
Methodology
Molecular
PCR
Test Description and clinical significance
Genes
BCR/ABL1 (t(9;22) Major (p210) breakpoints & Minor (p190) breakpoints
Disease:
B-cell lymphoblastic leukemia/lymphoma, and acute myeloid leukemia ,MPN
A polymerase chain reaction that is recommended for identification of BCR-ABL1 fusion genes. The BCR-ABL1 can produce proteins of differing sizes and weights, depending on where the break in chromosome 22 occurred. In CML, the breakpoint in BCR is almost always in the major breakpoint cluster region (M-BCR), leading to the production of BCR-ABL1 protein of a larger size (the protein is called p210). Breaks in the minor breakpoint cluster region (m-BCR) leads to a shorter fusion protein (called p190), which is most frequently associated with Ph chromosome-positive ALL. This tests proceeds onto detecting the minor break points when major break points are negative.
Specimen Requirements
Preparation:
PB, BM, RNA
Collection:
3ml EDTA, citrate (ACD) or Heparinized whole blood or bone marrow aspirates are transported at room temperature, received with 96 hr, refrigerated are stable for a week from the date of collection, Extracted RNA; a minimum volume of 20 μl at 100ng/μl
Stability:
96hours/4 to 7 days refrigerated
Unacceptable Conditions:
Clotted peripheral blood or bone marrow. Peripheral blood or bone marrow older than a week. Leukophoresis products. Improperly labeled specimens. Improperly stored RNA.
Storage & Transport
Blood or bone marrow specimens should be kept at room temperature and preferably received within 96 hrs of drawing, Previously extracted RNA is acceptable if stored at appropriate temperature short term at -20oC (up to two weeks) or long term at -80oC and should be transported on dry ice.
CPT(s)
81206, 81207
New York Approved
Yes
TAT
3 to 5
Requisition Type
Heme
Levels of Service
Full
DexS Code
NONE
Prefix
MI
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
