JAK-2 V617F Mutation Analysis
Unique Identifier:
ML-H016
Name on Requisition:
JAK-2 V617F
Product Line:
HEME
Panel Group:
PCR
Panel Name:
PCR–MPN/P.vera
Methodology
Molecular
PCR
Test Description and clinical significance
Genes
JAK-2 V617F
Disease:
myeloproliferative neoplasm, polycythemia vera, acute myeloid leukemia, overt primary myelofibrosis, and essential thrombocythemia
A polymerase chain reaction assay that detects V617F mutation . JAK2 is a kinase that is misregulated or mutated in a number of myeloproliferative diseases and cancers. The mutation V617F is the most clinically relevant variant, and is seen in around half of myeloproliferative disorders.
Specimen Requirements
Preparation:
PB, BM
Collection:
1-2 ml EDTA, citrate (ACD) or Heparinized whole blood or bone marrow, Extracted DNA A minimum of 20 μL of DNA with a concentration of 5ng/μL and an OD260/280 ratio of 1.7-1.9 obtained on a spectrophotometer is required
Stability:
96hours/4 to 7 days refrigerated
Unacceptable Conditions:
Unlabeled or improperly labeled samples, Samples older than stability date, Gross hemolysis, Improperly stored DNA.
Storage & Transport
Blood or bone marrow specimens should be kept at room temperature and preferably received within 96 hrs of drawing. Samples that are 4 to 7 days old should be refrigerated. Sufficient quantity of previously extracted DNA is acceptable if stored at appropriate temperature (short term at 2–8oC or long term at -15 to -25 oC) and should be transported on ice.
CPT(s)
81270
New York Approved
Yes
TAT
3
Requisition Type
Heme
Levels of Service
Full
DexS Code
ZB77X
Prefix
MI
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
