MPL Mutation Analysis
Unique Identifier:
ML-H017
Name on Requisition:
MPL
Product Line:
HEME
Panel Group:
PCR
Panel Name:
PCR–MPN
Methodology
Molecular
PCR
Test Description and clinical significance
Genes
MPL common variants (eg, W515A, W515K, W515L, W515R)
Disease:
MPN
The thrombopoietin receptor also known as the myeloproliferative leukemia protein or CD110 is a protein that is encoded by the MPL (myeloproliferative leukemia virus) oncogene. Specific mutations to this gene are associated with myelofibrosis and essential thrombocythemia.
Specimen Requirements
Preparation:
PB, BM
Collection:
1-2 ml EDTA, citrate (ACD) or Heparinized whole blood or bone marrow, Extracted DNA A minimum of 20 μL of DNA with a concentration of 5ng/μL and an OD260/280 ratio of 1.7-1.9 obtained on a spectrophotometer is required
Stability:
96hours/4 to 7 days refrigerated
Unacceptable Conditions:
Unlabeled or improperly labeled samples, Samples older than stability date, Gross hemolysis, Improperly stored DNA.
Storage & Transport
Blood or bone marrow specimens should be kept at room temperature and preferably received within 96 hrs of drawing. Samples that are 4 to 7 days old should be refrigerated. Sufficient quantity of previously extracted DNA is acceptable if stored at appropriate temperature (short term at 2–8oC or long term at -15 to -25 oC) and should be transported on ice.
CPT(s)
81338
New York Approved
Yes
TAT
3
Requisition Type
Heme
Levels of Service
Full
DexS Code
ZB77Q
Prefix
MI
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
