JAK2 Exon 12 Mutation Analysis
Unique Identifier:
ML-H018
Name on Requisition:
JAK2 Exon 12
Product Line:
HEME
Panel Group:
NGS
Panel Name:
P.vera
Methodology
Molecular
NGS
Test Description and clinical significance
Genes
JAK2 Exon 12
Disease:
PV
Mutations within exon 12 of the JAK2 gene occur in most cases of JAK2 V617F-mutation negative polycythemia vera. Testing for JAK2 exon 12 mutations may aid in the diagnosis of polycythemia vera, and is recommended in patients with JAK2 V617F-negative erythrocytosis. This is a second order test and should be used only following a JAK2 V617F-negative result.
Specimen Requirements
Preparation:
PB, BM
Collection:
at least 0.5 mL of EDTA, citrate (ACD) or Heparinized bone marrow and peripheral blood specimens, Extracted DNA A minimum of 20 μL of DNA with a concentration of 5ng/μL and an OD260/280 ratio of 1.7-1.9 obtained on a spectrophotometer is required
Stability:
96hours/4 to 7 days refrigerated
Unacceptable Conditions:
Unlabeled or improperly labeled samples, Samples older than stability date, Gross hemolysis, Improperly stored DNA.
Storage & Transport
Blood or bone marrow specimens should be kept at room temperature and preferably received within 96 hrs of drawing. Samples that are 4 to 7 days old should be refrigerated. Sufficient quantity of previously extracted DNA is acceptable if stored at appropriate temperature (short term at 2–8oC or long term at -15 to -25 oC) and should be transported on ice.
CPT(s)
81279
New York Approved
Yes
TAT
5
Requisition Type
Heme
Levels of Service
Full
DexS Code
ZB77P
Prefix
MI
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
