BRAF HCL
Unique Identifier:
ML-H020
Name on Requisition:
BRAF HCL
Product Line:
HEME
Panel Group:
PCR
Methodology
Molecular
PCR
Test Description and clinical significance
Genes
BRAF HCL (V600E)
Disease:
Hairy Cell Lukemia
BRAF V600E is the defining driver mutation in classic Hairy Cell Leukemia (HCL), present in the vast majority of cases and rarely seen in HCL-variant or other B-cell neoplasms. Detection of BRAF V600E supports diagnosis, informs prognosis, and may guide targeted therapy with BRAF/MEK inhibitors.
Specimen Requirements
Preparation:
PB, BM
Collection:
At least 0.5 ml EDTA, citrate (ACD) or Heparinized whole blood or bone marrow aspirates for DNA mutations
Stability:
96hours/4 to 7 days refrigerated
Unacceptable Conditions:
Specimen submitted is in the incorrect fixative or anticoagulant. Specimen received after the stability date. Gross hemolysis. Frozen specimen. Insufficient specimen. Unlabeled. Left un-refrigerated for an extended period.
Storage & Transport
Blood or bone marrow specimens should be kept at room temperature and preferably received within 96 hrs of drawing. Samples that are 4 to 7 days old should be refrigerated. Sufficient quantity of previously extracted DNA is acceptable if stored at appropriate temperature (short term at 2–8oC or long term at -15 to -25 oC) and should be transported on ice.
CPT(s)
81210
TAT
5
Requisition Type
Heme
Levels of Service
Full
DexS Code
Z05X2
Prefix
MI
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
