IDH1/IDH2
Unique Identifier:
ML-H022
Name on Requisition:
IDH1/IDH2
Product Line:
HEME
Panel Group:
NGS
Methodology
Molecular
NGS
Test Description and clinical significance
Genes
IDH1/IDH2
Disease:
AML
Isocitrate dehydrogenase 1 and 2 (IDH1 and IDH2) are metabolic enzymes frequently mutated in cancers such as acute myeloid leukemia, gliomas, and cholangiocarcinoma. These mutations produce the oncometabolite 2-hydroxyglutarate, driving tumorigenesis. This assay detects hotspot variants in IDH1 (codons 100, 132) and IDH2 (codons 140, 172), providing diagnostic, prognostic, and therapeutic insights.
Specimen Requirements
Preparation:
PB, BM
Collection:
At least 0.5 ml EDTA, citrate (ACD) or Heparinized whole blood or bone marrow aspirates for DNA mutations
Stability:
96hours/4 to 7 days refrigerated
Unacceptable Conditions:
Specimen submitted is in the incorrect fixative or anticoagulant. Specimen received after the stability date. Gross hemolysis. Frozen specimen. Insufficient specimen. Unlabeled. Left un-refrigerated for an extended period.
Storage & Transport
Blood or bone marrow specimens should be kept at room temperature and preferably received within 96 hrs of drawing. Samples that are 4 to 7 days old should be refrigerated. Sufficient quantity of previously extracted DNA is acceptable if stored at appropriate temperature (short term at 2–8oC or long term at -15 to -25 oC) and should be transported on ice.
CPT(s)
81120
New York Approved
Yes
TAT
5
Requisition Type
Heme
Levels of Service
Full
DexS Code
NONE
Prefix
MI
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
