BIRC3/MALT1
Methodology
FISH
Test Description and clinical significance
Probes
11q22.1-q22.2/18q21.31-q21.32
Disease:
B-cell non Hodgkin lymphoma (NHL), extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue, high grade ovarian serous adenocarcinoma
Designed to detect reciprocal translocation involving the human BIRC3 (API2) and MALT1 genes located on chromosome bands 11q22.2 and 18q21.32, respectively
Specimen Requirements
Collection:
The ordering pathologist will decide whether to order a conventional FISH preparation or a B-cell enrichment (BCE) FISH preparation.
Bone Marrow Aspirate: 1~2mL in Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. B-cell enrichment eligible.
Peripheral Blood: 2~5mL Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. B-cell enrichment eligible.
Fresh, Unfixed Tissue: Tissue in RPMI. A conventional FISH preparation will be performed as B-cell enrichment can not be performed on fresh tissues.
Fluids: Equal parts RPMI to specimen volume. A conventional FISH preparation will be performed as B-cell enrichment can not be performed on fresh fluid tissues.
Paraffin Block: H&E slide and paraffin block. B-cell enrichment can not be performed on paraffin embedded tissues.
Cut Slides: H&E slide and 1 unstained slide (cut at 4 microns) per probe. B-cell enrichment can not be performed on paraffin embedded tissues.
Stability:
120 Hours
Unacceptable Conditions:
DO NOT FREEZE
Storage & Transport
Room Temperature
CPT(s)
88377
New York Approved
YES
TAT
2 Days
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
