PML-RARA, Quant.
Unique Identifier:
ML-H027
Name on Requisition:
PML-RARA, Quant.
Product Line:
HEME
Panel Group:
PCR
Methodology
Molecular
PCR
Test Description and clinical significance
Genes
PML-RARA, Quant.
Disease:
Acute promyelocytic leukemia (APL)
PML-RARA is a fusion gene resulting from the t(15;17)(q24;q21) translocation, the hallmark of acute promyelocytic leukemia (APL). This rearrangement leads to production of the oncogenic PML-RARA fusion transcript, which blocks normal myeloid differentiation. Quantitative detection of PML-RARA transcripts by PCR is essential for diagnosis, baseline assessment, and monitoring of minimal residual disease (MRD) in APL. This assay measures PML-RARA transcript levels, enabling sensitive detection and longitudinal monitoring of treatment response and relapse.
Specimen Requirements
Preparation:
PB, BM
Collection:
At least 0.5 ml EDTA, citrate (ACD) or Heparinized whole blood or bone marrow aspirates for DNA mutations
Stability:
96hours/4 to 7 days refrigerated
Unacceptable Conditions:
Specimen submitted is in the incorrect fixative or anticoagulant. Specimen received after the stability date. Gross hemolysis. Frozen specimen. Insufficient specimen. Unlabeled. Left un-refrigerated for an extended period.
Storage & Transport
Blood or bone marrow specimens should be kept at room temperature and preferably received within 96 hrs of drawing. Samples that are 4 to 7 days old should be refrigerated. Sufficient quantity of previously extracted DNA is acceptable if stored at appropriate temperature (short term at 2–8oC or long term at -15 to -25 oC) and should be transported on ice.
CPT(s)
81315
New York Approved
NA
TAT
5
Requisition Type
Heme
Levels of Service
Full
DexS Code
Z05C3
Prefix
MI
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
