STAT3 and STAT5B (clonal LGL)
Unique Identifier:
ML-H032
Name on Requisition:
STAT3 and STAT5B (clonal LGL)
Panel Group:
NGS
Methodology
Molecular
NGS
Test Description and clinical significance
Genes
STAT3 and STAT5B
Disease:
large granular lymphocytic (LGL) leukemia
STAT3 and STAT5B encode transcription factors that mediate JAK–STAT signaling downstream of cytokine receptors. Somatic activating mutations in these genes are recurrently detected in clonal large granular lymphocytic (LGL) leukemia and contribute to abnormal T-cell or NK-cell proliferation and survival. Identification of STAT3 and STAT5B mutations supports the diagnosis of clonal LGL, provides prognostic information, and may guide therapeutic decisions. This assay detects clinically relevant hotspot variants in STAT3 and STAT5B.
Specimen Requirements
Preparation:
PB, BM
Collection:
At least 0.5 ml EDTA, citrate (ACD) or Heparinized whole blood or bone marrow aspirates for DNA mutations
Stability:
96hours/4 to 7 days refrigerated
Unacceptable Conditions:
Specimen submitted is in the incorrect fixative or anticoagulant. Specimen received after the stability date. Gross hemolysis. Frozen specimen. Insufficient specimen. Unlabeled. Left un-refrigerated for an extended period.
Storage & Transport
Blood or bone marrow specimens should be kept at room temperature and preferably received within 96 hrs of drawing. Samples that are 4 to 7 days old should be refrigerated. Sufficient quantity of previously extracted DNA is acceptable if stored at appropriate temperature (short term at 2–8oC or long term at -15 to -25 oC) and should be transported on ice.
CPT(s)
81405 & 81479
New York Approved
Yes
TAT
5
Requisition Type
Heme
Levels of Service
Full
DexS Code
NONE
Prefix
MI
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
