Probes
17p13.1/17p11.1-q11.1

Disease:
Multiple Myeloma, CLL, AML., ALL, Lymphoma, MDS

The p53 gene is a tumor suppressor gene, which usually controls cell growth and cell death.TP53 gene is located on the short arm of chromosome 17 (17p13.1) . Checking abnormal numbers of chromosome 17 and the deletion of chromosome 17p13 is important for therapeutic guidance and prognostic factor for many cancers. Germline mutation causes Li Fraumeni Syndrome. TP53 loss in patients with multiple myeloma is a late event, where it is seen as a marker of disease progression and is associated with a very poor prognosis

Collection:
The ordering pathologist will decide whether to order a conventional FISH preparation or a Plasma-cell enrichment (PCE) FISH preparation.
Bone Marrow Aspirate: 1~2mL in Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. Plasma cell enrichment eligible.
Peripheral Blood: 2~5mL Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. Plasma cell enrichment eligible ONLY IF circulating plasma cells are noted in PB.
Fresh, Unfixed Tissue: Tissue in RPMI. A conventional FISH preparation will be performed as plasma cell enrichment can not be performed on fresh tissues.
Fluids: Equal parts RPMI to specimen volume. A conventional FISH preparation will be performed as plasma cell enrichment can not be performed on fresh fluid tissues.
Paraffin Block: H&E slide and paraffin block. Plasma cell enrichment can not be performed on paraffin embedded tissues. Enumeration probe types (1p36/1q25, 13q14.2/13q34, CEN9, and TP53/CEN17) are not compatible with paraffin embedded tissues.
Cut Slides: H&E slide and 1 unstained slide (cut at 4 microns) per probe. Plasma cell enrichment can not be performed on paraffin embedded tissues. Enumeration probe types (1p36/1q25, 13q14.2/13q34, CEN9, and TP53/CEN17) are not compatible with paraffin embedded tissues.

Stability:
120 Hours/48 hours

Unacceptable Conditions:
DO NOT FREEZE