CEN9
Methodology
FISH
Test Description and clinical significance
Probes
9q12
Disease:
Multiple Myeloma, MPN
Designed to detect copy number variations of the 9q12 region on chromosome 9 and quantitative abnormalities of chromsome 9 .MM is associated with chromosome gain of ch 9.
NGS P.vera reflexes JAK2 V617F reflex to exons 12, 13 &15 are recommended
Specimen Requirements
Collection:
The ordering pathologist will decide whether to order a conventional FISH preparation or a Plasma-cell enrichment (PCE) FISH preparation.
Bone Marrow Aspirate: 1~2mL in Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. Plasma cell enrichment eligible.
Peripheral Blood: 2~5mL Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. Plasma cell enrichment eligible ONLY IF circulating plasma cells are noted in PB.
Fresh, Unfixed Tissue: Tissue in RPMI. A conventional FISH preparation will be performed as plasma cell enrichment can not be performed on fresh tissues.
Fluids: Equal parts RPMI to specimen volume. A conventional FISH preparation will be performed as plasma cell enrichment can not be performed on fresh fluid tissues.
Paraffin Block: H&E slide and paraffin block. Plasma cell enrichment can not be performed on paraffin embedded tissues. Enumeration probe types (1p36/1q25, 13q14.2/13q34, CEN9, and TP53/CEN17) are not compatible with paraffin embedded tissues.
Cut Slides: H&E slide and 1 unstained slide (cut at 4 microns) per probe. Plasma cell enrichment can not be performed on paraffin embedded tissues. Enumeration probe types (1p36/1q25, 13q14.2/13q34, CEN9, and TP53/CEN17) are not compatible with paraffin embedded tissues.
Stability:
120 Hours/48 hours
Unacceptable Conditions:
DO NOT FREEZE
Storage & Transport
Room Temperature/Refrigerated (2–8°C )
CPT(s)
88368 for intial single probe & 88369 for each additional single probe
New York Approved
YES
TAT
2 Days
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
