MYB/CEN6
Methodology
FISH
Test Description and clinical significance
Probes
6p11.1-q11/6q23.2-23.3
Disease:
Waldenstrom Macroglobinemia, leukemia, lymphoma, malignant melanoma
Qualitative detection of amplifications involving the human MYB gene on chromosome 6 and the numerical abnormalities of chromosome 6. MYB gene encodes a protein with three HTH DNA-binding domains that functions as a transcription regulator. This protein plays an essential role in the regulation of hematopoiesis. This gene may be aberrently expressed or rearranged or undergo translocation in_some cancers and is considered to be an oncogene.
MYD88 testing is recommended
Specimen Requirements
Collection:
The ordering pathologist will decide whether to order a conventional FISH preparation or a B-cell enrichment (BCE) FISH preparation.
Bone Marrow Aspirate: 1~2mL in Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. B-cell enrichment eligible.
Peripheral Blood: 2~5mL Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. B-cell enrichment eligible.
Fresh, Unfixed Tissue: Tissue in RPMI. A conventional FISH preparation will be performed as B-cell enrichment can not be performed on fresh tissues.
Fluids: Equal parts RPMI to specimen volume. A conventional FISH preparation will be performed as B-cell enrichment can not be performed on fresh fluid tissues.
Paraffin Block: H&E slide and paraffin block. B-cell enrichment can not be performed on paraffin embedded tissues.
Cut Slides: H&E slide and 1 unstained slide (cut at 4 microns) per probe. B-cell enrichment can not be performed on paraffin embedded tissues.
Stability:
120 Hours
Unacceptable Conditions:
DO NOT FREEZE
Storage & Transport
Room Temperature
CPT(s)
88377, 88112
New York Approved
YES
TAT
2 Days
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
