Karyotyping Lymphoid - B-Cell
Methodology
Karyotyping
Test Description and clinical significance
Disease:
Subtyping of lymphomas, leukemias and solid tumours for the purpose of exact diagnosis, treatment stratification and prognosis, prenatal diagnosis, investigation of repeat pregnancy loss, investigation of a possible syndromes.
Karyotyping is the process of pairing and ordering all the chromosomes of an organism, thus providing a genome-wide snapshot of an individual's chromosomes. Karyotypes are prepared using standardized staining procedures that reveal characteristic structural features for each chromosome. Clinical cytogeneticists analyze human karyotypes to detect gross genetic changes—anomalies involving several megabases or more of DNA. Karyotypes can reveal changes in chromosome number, and areful analysis of karyotypes can also reveal more subtle structural changes, such as chromosomal deletions, duplications, translocations, insertions,or inversions. Culture done and checked at day 1 and day 5
Specimen Requirements
Collection:
Green top (Sodium Heparin), Bone Marrow is ≥1.5 mL and the Peripheral blood is ≥2 mL if 10% or more blasts are present
Stability:
Up to 72 hours
Unacceptable Conditions:
No Frozen, Hemolyzed, Washed, in Syringes, in broken container or in different anti-coagulant other than sodium heparin.
Storage & Transport
Room Temperature
CPT(s)
88237, 88264, 88291, some cases may require additional studies requiring additional CPT codes.
New York Approved
YES
TAT
6 Days
*The CPT codes provided are for informational purposes only and are based on AMA guidelines The billing party is solely responsible for correct CPT coding.
